| 1 | Gianna GyurichPlace 1 · Grade 11 · Bishop Verot | 11 | Bishop Verot | 20:17.6 |
| 2 | Livi FeyereisenPlace 2 · Grade 12 · Canterbury (Fort Myers) | 12 | Canterbury (Fort Myers) | 20:41.1 |
| 3 | Jayden WilkinsPlace 3 · Grade 10 · Southwest Florida Christian Acad | 10 | Southwest Florida Christian Acad | 20:50.3 |
| 4 | Lillian LampittPlace 4 · Grade 9 · Bishop Verot | 9 | Bishop Verot | 21:18.8 |
| 5 | Anna Kate CastoPlace 5 · Grade 10 · Community School | 10 | Community School | 21:40.7 |
| 6 | Rose O'MalleyPlace 6 · Grade 11 · Community School | 11 | Community School | 22:43.5 |
| 7 | Brooke ShefskyPlace 7 · Grade 10 · St John Neumann | 10 | St John Neumann | 22:55.3 PR |
| 8 | Addison MatthewsPlace 8 · Grade 8 · Southwest Florida Christian Acad | 8 | Southwest Florida Christian Acad | 23:02.6 |
| 9 | Sidney DawickPlace 9 · Grade 10 · Canterbury (Fort Myers) | 10 | Canterbury (Fort Myers) | 23:07.9 |
| 10 | Claire DawickPlace 10 · Grade 11 · Canterbury (Fort Myers) | 11 | Canterbury (Fort Myers) | 23:48.8 |
| 11 | Lana SchmidtPlace 11 · Grade 10 · Bishop Verot | 10 | Bishop Verot | 24:10.9 |
| 12 | Olivia AntoonPlace 12 · Grade 9 · Bishop Verot | 9 | Bishop Verot | 24:29.0 |
| 13 | Zoe McCurryPlace 13 · Grade 10 · Bishop Verot | 10 | Bishop Verot | 24:36.9 |
| 14 | Lea ScottiPlace 14 · Grade 12 · Bishop Verot | 12 | Bishop Verot | 24:39.1 |
| 15 | Jackie WentworthPlace 15 · Grade 12 · First Baptist | 12 | First Baptist | 24:50.7 |
| 16 | Jade SchmidtPlace 16 · Grade 9 · Bishop Verot | 9 | Bishop Verot | 25:08.2 |
| 17 | Anna RashidPlace 17 · Grade 11 · First Baptist | 11 | First Baptist | 25:11.4 |
| 18 | Katie WisnerPlace 18 · Grade 9 · Evangelical Christian | 9 | Evangelical Christian | 25:26.7 |
| 19 | Brittan SpencerPlace 19 · Grade 11 · First Baptist | 11 | First Baptist | 25:27.2 |
| 20 | Brooke SpencerPlace 20 · Grade 12 · First Baptist | 12 | First Baptist | 25:28.9 |
| 21 | Christine FrostmanPlace 21 · Grade 12 · Evangelical Christian | 12 | Evangelical Christian | 25:49.4 |
| 22 | Stella MassollPlace 22 · Grade 10 · Community School | 10 | Community School | 25:50.0 |
| 23 | Hanna HagenbucklePlace 23 · Grade 12 · Community School | 12 | Community School | 25:50.6 |
| 24 | Madilyn PicascioPlace 24 · Grade 11 · First Baptist | 11 | First Baptist | 25:51.3 |
| 25 | Ashley VillaflorPlace 25 · Grade 10 · Evangelical Christian | 10 | Evangelical Christian | 26:04.2 |
| 26 | Isabella PierrePlace 26 · Grade 9 · Bishop Verot | 9 | Bishop Verot | 26:08.3 |
| 27 | Isabella HobtPlace 27 · Grade 12 · Canterbury (Fort Myers) | 12 | Canterbury (Fort Myers) | 26:12.3 |
| 28 | Alden ConlonPlace 28 · Grade 10 · St John Neumann | 10 | St John Neumann | 26:23.1 |
| 29 | Emma MorrellPlace 29 · Grade 9 · Southwest Florida Christian Acad | 9 | Southwest Florida Christian Acad | 26:27.9 |
| 30 | Cora WellsPlace 30 · Grade 11 · First Baptist | 11 | First Baptist | 26:33.4 |
| 31 | Flora BarnettPlace 31 · Grade 9 · Canterbury (Fort Myers) | 9 | Canterbury (Fort Myers) | 26:45.5 |
| 32 | Giuliana MolinoPlace 32 · Grade 10 · Southwest Florida Christian Acad | 10 | Southwest Florida Christian Acad | 27:05.5 |
| 33 | Drea RosePlace 33 · Grade 9 · Evangelical Christian | 9 | Evangelical Christian | 27:15.4 |
| 34 | WILSON ReaganPlace 34 · Grade 9 · Evangelical Christian | 9 | Evangelical Christian | 27:28.2 |
| 35 | Mia McmastersPlace 35 · Grade 12 · Bishop Verot | 12 | Bishop Verot | 27:38.4 |
| 36 | Aubry LairedPlace 36 · Grade 6 · Evangelical Christian | 6 | Evangelical Christian | 28:09.0 PR |
| 37 | Reese NoelPlace 37 · Grade 12 · First Baptist | 12 | First Baptist | 28:13.9 |
| 38 | Olivia MoorePlace 38 · Grade 10 · Bishop Verot | 10 | Bishop Verot | 28:14.4 |
| 39 | Emma MooneyPlace 39 · Grade 9 · Evangelical Christian | 9 | Evangelical Christian | 28:25.6 |
| 40 | Lydia YarnellPlace 40 · Grade 10 · Southwest Florida Christian Acad | 10 | Southwest Florida Christian Acad | 28:27.7 |
| 41 | Yana RothmanPlace 41 · Grade 10 · Seacrest Country Day | 10 | Seacrest Country Day | 28:29.8 |
| 42 | Lauren TaylorPlace 42 · Grade 12 · Evangelical Christian | 12 | Evangelical Christian | 28:43.4 |
| 43 | Mia WestPlace 43 · Grade 12 · Canterbury (Fort Myers) | 12 | Canterbury (Fort Myers) | 29:40.7 |
| 44 | Ruthee WisnerPlace 44 · Grade 12 · Evangelical Christian | 12 | Evangelical Christian | 29:51.1 |
| 45 | Riley SmithPlace 45 · Grade 9 · St John Neumann | 9 | St John Neumann | 30:33.0 |
| 46 | Josie PopePlace 46 · Grade 10 · Southwest Florida Christian Acad | 10 | Southwest Florida Christian Acad | 30:35.5 |
| 47 | Rosina ManfrediPlace 47 · Grade 10 · St John Neumann | 10 | St John Neumann | 31:24.0 |
| 48 | Micaela RuizPlace 48 · Grade 12 · Southwest Florida Christian Acad | 12 | Southwest Florida Christian Acad | 31:27.1 |
| 49 | Addison RoyerPlace 49 · Grade 9 · Bishop Verot | 9 | Bishop Verot | 33:15.5 |
| 50 | Carina ManfrediPlace 50 · Grade 10 · St John Neumann | 10 | St John Neumann | 35:19.3 |
| 51 | Sarah GonzalezPlace 51 · Grade 9 · Bishop Verot | 9 | Bishop Verot | 36:10.4 |
| 52 | Reagan MahoneyPlace 52 · Grade 9 · Southwest Florida Christian Acad | 9 | Southwest Florida Christian Acad | 40:59.2 |
