| 1 | Austin SandersPlace 1 · Michigan All-Stars | — | Michigan All-Stars | 22.79 |
| 2 | William Askew IIPlace 2 · Maximum Output | — | Maximum Output | 23.59 |
| 3 | Chris NorrisPlace 3 · Detroit Cheetah Track Clu | — | Detroit Cheetah Track Clu | 23.76 |
| 4 | Nolen MitchellPlace 4 · Albion TC | — | Albion TC | 23.79 |
| 5 | Wan'ya WilliamsPlace 5 · Michigan All-Stars | — | Michigan All-Stars | 23.82 |
| 5 | Damonta MaddenPlace 5 · Tempo | — | Tempo | 23.82 |
| 7 | Aaron DavisPlace 7 · Detroit Cheetah Track Clu | — | Detroit Cheetah Track Clu | 23.85 |
| 8 | Jeffrey JohnsonPlace 8 · Elite Performance | — | Elite Performance | 23.94 |
| 9 | Travis SteinbackPlace 9 · New Breed Track Club | — | New Breed Track Club | 23.95 |
| 10 | Matthew FalconPlace 10 · Michigan All-Stars | — | Michigan All-Stars | 23.99 |
| 11 | Garrick RoemerPlace 11 · Spirit of Pre | — | Spirit of Pre | 24.00 |
| 11 | Omar RussellPlace 11 · Michigan All-Stars | — | Michigan All-Stars | 24.00 |
| 11 | Kabine DianePlace 11 · Michigan All-Stars | — | Michigan All-Stars | 24.00 |
| 14 | Ryan BrownPlace 14 · Elite Performance | — | Elite Performance | 24.01 |
| 14 | Kendall ThompsonPlace 14 · Michigan All-Stars | — | Michigan All-Stars | 24.01 |
| 16 | Payton WheelerPlace 16 · Michigan All Stars | — | Michigan All Stars | 24.02 |
| 16 | Dewan Olive-AbinojarPlace 16 · Michigan All-Stars | — | Michigan All-Stars | 24.02 |
| 18 | Darius WallacePlace 18 · Michigan All-Stars | — | Michigan All-Stars | 24.04 |
| 19 | Ronnie Craft-BushPlace 19 · Elite Performance | — | Elite Performance | 24.06 |
| 20 | Miral HoliPlace 20 · Detroit Cheetah Track Clu | — | Detroit Cheetah Track Clu | 24.07 |
| 21 | Brandon PhillipsPlace 21 · Michigan All-Stars | — | Michigan All-Stars | 24.11 |
| 22 | Alton YarboroughPlace 22 · Maximum Output | — | Maximum Output | 24.28 |
| 23 | Joe PreniPlace 23 · Unattached | — | Unattached | 24.29 |
| 24 | AJ GoffPlace 24 · Elite Performance | — | Elite Performance | 24.36 |
| 25 | Nick ReisPlace 25 · Elite Performance | — | Elite Performance | 24.41 |
| 26 | Eddie CodringtonPlace 26 · Ann Arbor Track Club | — | Ann Arbor Track Club | 24.46 |
| 27 | Malik HallPlace 27 · Michigan All-Stars | — | Michigan All-Stars | 24.48 |
| 27 | Duane BoydPlace 27 · Michigan All-Stars | — | Michigan All-Stars | 24.48 |
| 29 | Tyrus StevensPlace 29 · Michigan All-Stars | — | Michigan All-Stars | 24.50 |
| 30 | Kenning BrichfordPlace 30 · 6-Mile Track Club | — | 6-Mile Track Club | 24.54 |
| 31 | Daniel HuganPlace 31 · Michigan All Stars | — | Michigan All Stars | 24.55 |
| 32 | Brian MatthewPlace 32 · Tempo | — | Tempo | 24.56 |
| 33 | Tyrone Coleman Jr.Place 33 · Michigan All-Stars | — | Michigan All-Stars | 24.63 |
| 34 | Dominick LemaniousPlace 34 · Motor City Track Club | — | Motor City Track Club | 24.67 |
| 34 | DeVaun WhitePlace 34 · Michigan Accelerators | — | Michigan Accelerators | 24.67 |
| 36 | Parker WheathexPlace 36 · Tempo | — | Tempo | 24.68 |
| 37 | Damian MenochPlace 37 · Unattached | — | Unattached | 24.70 |
| 38 | Quinton DavisPlace 38 · Michigan Accelerators | — | Michigan Accelerators | 24.75 |
| 38 | Ray BufordPlace 38 · New Breed Track Club | — | New Breed Track Club | 24.75 |
| 40 | Jordan BillingsleaPlace 40 · New Breed Track Club | — | New Breed Track Club | 24.77 |
| 41 | Jordan MasseyPlace 41 · Detroit Cheetah Track Clu | — | Detroit Cheetah Track Clu | 24.79 |
| 42 | Illia PanevPlace 42 · Elite Performance | — | Elite Performance | 24.86 |
| 43 | Derris CleaverPlace 43 · Michigan Accelerators | — | Michigan Accelerators | 24.89 |
| 44 | Jamil BanksPlace 44 · Tempo | — | Tempo | 24.93 |
| 45 | Christian VostersPlace 45 · Unattached | — | Unattached | 24.95 |
| 46 | Sherrard JacksonPlace 46 · Michigan Accelerators | — | Michigan Accelerators | 24.97 |
| 47 | Jordan BankerPlace 47 · Southfield Mustangs | — | Southfield Mustangs | 25.09 |
| 48 | Nick PodulkaPlace 48 · 6-Mile Track Club | — | 6-Mile Track Club | 25.12 |
| 49 | KJ OsbornPlace 49 · Michigan All-Stars | — | Michigan All-Stars | 25.14 |
| 50 | Gabe HadazePlace 50 · Invictus TC | — | Invictus TC | 25.22 |
| 50 | Alex CookenmasterPlace 50 · Tempo | — | Tempo | 25.22 |
| 52 | Alex JupuvenkPlace 52 · Unattached | — | Unattached | 25.29 |
| 53 | Marcus ThomsonPlace 53 · Michigan Accelerators | — | Michigan Accelerators | 25.32 |
| 54 | Kevin RoseniPlace 54 · The U | — | The U | 25.37 |
| 55 | Drake GauthierPlace 55 · Tempo | — | Tempo | 25.44 |
| 56 | Tarentay WashingtonPlace 56 · Elite Performance | — | Elite Performance | 25.48 |
| 57 | Dashawn TolbertPlace 57 · Elite Performance | — | Elite Performance | 25.50 |
| 58 | William ParkerPlace 58 · Michigan Accelerators | — | Michigan Accelerators | 25.55 |
| 59 | Matthew JordanPlace 59 · Michigan Accelerators | — | Michigan Accelerators | 25.59 |
| 60 | Alan HurtlinzPlace 60 · Unattached | — | Unattached | 25.60 |
| 61 | James AbbeyPlace 61 · The U | — | The U | 25.67 |
| 62 | Aaron HathornePlace 62 · Lake Erie Striders | — | Lake Erie Striders | 25.72 |
| 63 | Daniel O'NeillPlace 63 · New Breed Track Club | — | New Breed Track Club | 25.74 |
| 64 | Bilal HowardPlace 64 · Ypsilanti Track Club | — | Ypsilanti Track Club | 25.76 |
| 65 | Rob DemmerPlace 65 · Dearborn Track Club | — | Dearborn Track Club | 25.78 |
| 66 | Glaine StewartPlace 66 · Unattached | — | Unattached | 25.95 |
| 67 | John FisherPlace 67 · Ann Arbor Track Club | — | Ann Arbor Track Club | 25.97 |
| 68 | Christina AkersPlace 68 · Unattached | — | Unattached | 26.03 |
| 69 | Alex GolinshiPlace 69 · Breen TC | — | Breen TC | 26.20 |
| 70 | Robert HarrisPlace 70 · Unattached | — | Unattached | 26.23 |
| 71 | Zac FreelandPlace 71 · The U | — | The U | 26.38 |
| 71 | Eric PurrPlace 71 · Unattached | — | Unattached | 26.38 |
| 73 | Tyler MackPlace 73 · Unattached | — | Unattached | 26.42 |
| 73 | Niko FrancePlace 73 · Michigan Accelerators | — | Michigan Accelerators | 26.42 |
| 75 | Jeff DunaghPlace 75 · Unattached | — | Unattached | 26.53 |
| 76 | Ty NelsonPlace 76 · Unattached | — | Unattached | 26.66 |
| 77 | Christopher RichtoPlace 77 · Unattached | — | Unattached | 26.72 |
| 77 | Quin FerberPlace 77 · Breen TC | — | Breen TC | 26.72 |
| 79 | Alex DeherderPlace 79 · Southfield Mustangs | — | Southfield Mustangs | 26.73 |
| 80 | Joel SiedellPlace 80 · Invictus TC | — | Invictus TC | 26.76 |
| 81 | Jesston WilliamsPlace 81 · Unattached | — | Unattached | 26.80 |
| 81 | Rex WagnerPlace 81 · Lake Erie Striders | — | Lake Erie Striders | 26.80 |
| 83 | Tanner DingmanPlace 83 · Invictus TC | — | Invictus TC | 26.89 |
| 84 | Ron PennyPlace 84 · Unattached | — | Unattached | 27.24 |
| 85 | Joe PlennerPlace 85 · Invictus TC | — | Invictus TC | 27.31 |
| 86 | John ChopsonPlace 86 · Air | — | Air | 27.61 |
| 87 | Adam RegalskiPlace 87 · Breen TC | — | Breen TC | 27.78 |
| 88 | Kenny McDowellPlace 88 · Unattached | — | Unattached | 27.88 |
| 89 | Michael MattiocciPlace 89 · Lake Erie Striders | — | Lake Erie Striders | 28.38 |
| 90 | Bruce PickettPlace 90 · New Breed Track Club | — | New Breed Track Club | 28.58 |
| 91 | Jayla LeonardPlace 91 · Breen TC | — | Breen TC | 28.72 |
| 92 | Jordan YoungPlace 92 · New Breed Track Club | — | New Breed Track Club | 29.61 |
| 93 | Kyle MartinPlace 93 · Ann Arbor Track Club | — | Ann Arbor Track Club | 29.69 |
| 94 | Dylan HarrisPlace 94 · Ann Arbor Track Club | — | Ann Arbor Track Club | 29.72 |
| 95 | Codie JamesPlace 95 · Air | — | Air | 29.88 |
| 96 | Jimmie FrancoisPlace 96 · Ann Arbor Track Club | — | Ann Arbor Track Club | 33.67 |
| 97 | Colin MiedenPlace 97 · Air | — | Air | 34.18 |
| 98 | Walter HerndonPlace 98 · Inkster Jaguars Track Clu | — | Inkster Jaguars Track Clu | 36.76 |
| 99 | Bobby WellsPlace 99 · Inkster Jaguars Track Clu | — | Inkster Jaguars Track Clu | 48.16 |