| 1. | Makenzie FallonPlace 1. · Grade 12 · Fenwick | 12 | Fenwick | 1:00.30a | 10 |
| 2. | Sophia TebbePlace 2. · Grade 9 · Fenwick | 9 | Fenwick | 1:01.06a | 8 |
| 3. | Priscilla MartinezPlace 3. · Grade 10 · Monroe | 10 | Monroe | 1:01.27a | 6 |
| 4. | Laila BlackPlace 4. · Grade 11 · Franklin | 11 | Franklin | 1:06.10a PR | 5 |
| 5. | Keyarah MccurdyPlace 5. · Grade 11 · Middletown | 11 | Middletown | 1:07.08a | 4 |
| 6. | Sarah HollinsPlace 6. · Grade 12 · Dominion Academy | 12 | Dominion Academy | 1:07.70a | 3 |
| 7. | Sienna FintonPlace 7. · Grade 11 · Franklin | 11 | Franklin | 1:08.01a PR | 2 |
| 8. | Reagan PieschelPlace 8. · Grade 10 · Franklin | 10 | Franklin | 1:08.69a | 1 |
| 9. | Rylyn ReynoldsPlace 9. · Grade 10 · Badin | 10 | Badin | 1:09.15a PR | — |
| 10. | Watkins DaiyaPlace 10. · Grade 12 · Middletown | 12 | Middletown | 1:09.33a | — |
| 11. | Cicillia SnyderPlace 11. · Grade 10 · McNicholas | 10 | McNicholas | 1:09.82a | — |
| 12. | Summer ShermanPlace 12. · Grade 9 · Waynesville | 9 | Waynesville | 1:11.05a | — |
| 13. | Lindsey BrownPlace 13. · Grade 11 · McNicholas | 11 | McNicholas | 1:12.26a | — |
| 14. | Amani LackeyPlace 14. · Grade 10 · Mt. Healthy | 10 | Mt. Healthy | 1:12.65a PR | — |
| 15. | Vivian ZettlerPlace 15. · Grade 12 · Badin | 12 | Badin | 1:12.80a PR | — |
| 16. | Natalie CoxPlace 16. · Grade 12 · McNicholas | 12 | McNicholas | 1:15.43a PR | — |
| 17. | Jessica HammondPlace 17. · Grade 10 · Alter | 10 | Alter | 1:16.80a | — |
| 18. | Lily GonzalezPlace 18. · Grade 10 · Waynesville | 10 | Waynesville | 1:16.86a | — |
| 19. | Carissa GallierPlace 19. · Grade 9 · Mars Hill Academy | 9 | Mars Hill Academy | 1:17.10a PR | — |
| 20. | Anna DraytonPlace 20. · Grade 9 · Alter | 9 | Alter | 1:17.75a | — |
| 21. | Grace RooneyPlace 21. · Grade 11 · Alter | 11 | Alter | 1:18.01a PR | — |
| 22. | Ava MenningPlace 22. · Grade 11 · Newport Central Catholic | 11 | Newport Central Catholic | 1:18.49a PR | — |
| 23. | Emilia WatkinsPlace 23. · Grade 9 · Alter | 9 | Alter | 1:18.56a | — |
| 24. | Kaelee DuScheidPlace 24. · Grade 9 · Royalmont Academy | 9 | Royalmont Academy | 1:18.96a | — |
| 25. | Clare GiffenPlace 25. · Grade 11 · Badin | 11 | Badin | 1:20.44a PR | — |
| 26. | Dailys HernandezPlace 26. · Grade 9 · Middletown | 9 | Middletown | 1:21.10a | — |
| 27. | Caroline KreylingPlace 27. · Grade 9 · MVCA | 9 | MVCA | 1:21.47a | — |
| 28. | Ainhara Montilla SierraPlace 28. · Grade 9 · Middletown | 9 | Middletown | 1:21.96a PR | — |
| 29. | Mia MacarioPlace 29. · Grade 10 · Middletown | 10 | Middletown | 1:22.68a PR | — |
| 30. | Rachel BurkhartPlace 30. · Grade 9 · Newport Central Catholic | 9 | Newport Central Catholic | 1:22.88a PR | — |
| 31. | Clara McConnellPlace 31. · Grade 9 · MVCA | 9 | MVCA | 1:23.73a | — |
| 32. | Elizabeth ThomasPlace 32. · Grade 9 · MVCA | 9 | MVCA | 1:23.79a | — |
| 33. | Lise Hernandez RodasPlace 33. · Grade 9 · Middletown | 9 | Middletown | 1:24.75a PR | — |
| 34. | Kenady EdwardsPlace 34. · Grade 9 · Middletown | 9 | Middletown | 1:28.08a | — |
| 35. | Dar'niya SmithPlace 35. · Grade 11 · Western Hills | 11 | Western Hills | 1:30.03a | — |
| 36. | Melissa SomenutsePlace 36. · Grade 11 · Middletown | 11 | Middletown | 1:35.98a PR | — |
