| 1. | Lydia VincentPlace 1. · Grade 11 · Zephyrhills | 11 | Zephyrhills | 2:17.30a PR |
| 2. | Ainsley AeschlemanPlace 2. · Grade 12 · George Steinbrenner | 12 | George Steinbrenner | 2:25.40a PR |
| 3. | Blake PeiferPlace 3. · Grade 11 · George Steinbrenner | 11 | George Steinbrenner | 2:25.73a |
| 4. | Naylani SmithPlace 4. · Grade 12 · East Bay | 12 | East Bay | 2:25.90a PR |
| 5. | Ramya AtienzaPlace 5. · Grade 12 · Winthrop College Prep Academy | 12 | Winthrop College Prep Academy | 2:26.08a PR |
| 6. | Allison WellsPlace 6. · Grade 11 · Cypress Creek (Wesley Chapel) | 11 | Cypress Creek (Wesley Chapel) | 2:33.79a PR |
| 7. | Malina BunwatPlace 7. · Grade 8 · Angeline Academy of Innovation | 8 | Angeline Academy of Innovation | 2:34.11a PR |
| 8. | Julia DillinghamPlace 8. · Grade 11 · Out-of-Door Academy | 11 | Out-of-Door Academy | 2:34.25a |
| 9. | Cecilia MichelPlace 9. · Grade 12 · Wesley Chapel | 12 | Wesley Chapel | 2:37.44a |
| 10. | Lisa MuehlfellnerPlace 10. · Grade 12 · Palm Harbor University | 12 | Palm Harbor University | 2:37.90a |
| 11. | Maddie MaldonadoPlace 11. · Grade 12 · Palm Harbor University | 12 | Palm Harbor University | 2:42.56a |
| 12. | Mia MurrayPlace 12. · Grade 10 · Cypress Creek (Wesley Chapel) | 10 | Cypress Creek (Wesley Chapel) | 2:43.40a |
| 13. | Brianna LachapellePlace 13. · Grade 12 · Alonso | 12 | Alonso | 2:43.94a |
| 14. | Mya GruberPlace 14. · Grade 9 · Out-of-Door Academy | 9 | Out-of-Door Academy | 2:46.51a |
| 15. | Angelina CacciolaPlace 15. · Grade 12 · River Ridge | 12 | River Ridge | 2:47.67a PR |
| 16. | Moriah TolesPlace 16. · Grade 6 · Carrollwood Day | 6 | Carrollwood Day | 2:48.08a |
| 17. | Ava SpearinPlace 17. · Grade 12 · Wiregrass Ranch | 12 | Wiregrass Ranch | 2:48.27a |
| 18. | Malaina BricknerPlace 18. · Grade 11 · Carrollwood Day | 11 | Carrollwood Day | 2:49.85a |
| 19. | Olivia LopezPlace 19. · Grade 12 · Land O'Lakes | 12 | Land O'Lakes | 2:51.07a |
| 20. | Savannah BakerPlace 20. · Grade 10 · Robinson | 10 | Robinson | 2:53.00a PR |
| 21. | Avery BrewerPlace 21. · Grade 11 · Springstead | 11 | Springstead | 2:55.89a |
| 22. | Anika SankaPlace 22. · Grade 12 · Robinson | 12 | Robinson | 2:56.86a |
| 23. | Madelyn MountsPlace 23. · Grade 9 · River Ridge | 9 | River Ridge | 2:57.22a |
| 24. | Breille BakerPlace 24. · Grade 8 · Bell Creek | 8 | Bell Creek | 3:00.97a |
| 25. | Bryanna NicholsPlace 25. · Grade 10 · J.W. Mitchell | 10 | J.W. Mitchell | 3:01.76a |
| 26. | Alyssa HoytPlace 26. · Grade 12 · Springstead | 12 | Springstead | 3:02.70a |
| 27. | Olivia AllenPlace 27. · Grade 12 · Springstead | 12 | Springstead | 3:12.71a |
| 28. | Amelia SommersPlace 28. · Grade 11 · Carrollwood Day | 11 | Carrollwood Day | 3:18.35a |
| 29. | Manuela Porras RamirezPlace 29. · Grade 10 · Alonso | 10 | Alonso | 3:19.40a PR |
| 30. | Julianna TranPlace 30. · Grade 10 · Alonso | 10 | Alonso | 3:22.10a |
| 31. | Reyin LubaPlace 31. · Grade 10 · Bishop McLaughlin | 10 | Bishop McLaughlin | 3:31.08a |
| 32. | Madison VargasPlace 32. · Grade 11 · Anclote | 11 | Anclote | 3:48.76a |
| 33. | Isabella Beato-MolinaPlace 33. · Grade 9 · Bell Creek | 9 | Bell Creek | 3:55.47a PR |
| 34. | Adriana PalmeroPlace 34. · Grade 10 · Bishop McLaughlin | 10 | Bishop McLaughlin | 4:10.08a PR |
| 35. | Fairy BarotPlace 35. · Grade 10 · Dr. Kiran C. Patel | 10 | Dr. Kiran C. Patel | 4:54.38a PR |
