| 1. | Rodney TownsendPlace 1. · Grade 12 · Oakleaf | 12 | Oakleaf | 49.65a |
| 2. | LaWarren NewtonPlace 2. · Grade 12 · Columbia (Lake City) | 12 | Columbia (Lake City) | 50.28a |
| 3. | Lavarius JonesPlace 3. · Grade 11 · Orange Park | 11 | Orange Park | 51.29a |
| 4. | Christian BradstreetPlace 4. · Grade 10 · Oakleaf | 10 | Oakleaf | 52.26a |
| 5. | Daniel JohnsonPlace 5. · Grade 12 · Middleburg | 12 | Middleburg | 52.48a |
| 6. | Michard GeffrardPlace 6. · Grade 11 · Ridgeview | 11 | Ridgeview | 53.50a |
| 7. | Mekah Combs-TurnerPlace 7. · Grade 11 · Columbia (Lake City) | 11 | Columbia (Lake City) | 53.99a |
| 8. | Damarcus MoorePlace 8. · Grade 10 · Columbia (Lake City) | 10 | Columbia (Lake City) | 54.46a |
| 9. | Kendrick MunnerlynPlace 9. · Grade 12 · Bradford | 12 | Bradford | 54.66a |
| 10. | Sawyer HamptonPlace 10. · Grade 11 · Middleburg | 11 | Middleburg | 55.09a |
| 11. | Delmar WhitePlace 11. · Grade 11 · Fleming Island | 11 | Fleming Island | 55.17a |
| 12. | Jamie LopeyPlace 12. · Grade 10 · Bradford | 10 | Bradford | 55.18a PR |
| 13. | Luke LairdPlace 13. · Grade 12 · Fleming Island | 12 | Fleming Island | 56.17a PR |
| 14. | Branden TysonPlace 14. · Grade 11 · Duval Charter | 11 | Duval Charter | 56.29a PR |
| 15. | Karsen MonroePlace 15. · Grade 12 · Ridgeview | 12 | Ridgeview | 56.33a |
| 16. | Orion TishPlace 16. · Grade 10 · Fleming Island | 10 | Fleming Island | 56.33a PR |
| 17. | David WilsonPlace 17. · Grade 11 · St Joseph Academy | 11 | St Joseph Academy | 56.36a |
| 18. | Aviance MclaurinPlace 18. · Grade 10 · University Christian | 10 | University Christian | 56.40a PR |
| 19. | Corbin EllisPlace 19. · Grade 12 · University Christian | 12 | University Christian | 56.45a PR |
| 20. | Alexander KuilanPlace 20. · Grade 9 · Duval Charter | 9 | Duval Charter | 56.66a |
| 21. | Dayton JacksonPlace 21. · Grade 11 · Oakleaf | 11 | Oakleaf | 56.68a PR |
| 22. | Terrod LloydPlace 22. · Grade 10 · Orange Park | 10 | Orange Park | 56.89a |
| 23. | Jayden WessonPlace 23. · Grade 11 · Oakleaf | 11 | Oakleaf | 56.90a |
| 24. | Daniel GayPlace 24. · Grade 11 · Orange Park | 11 | Orange Park | 56.96a PR |
| 25. | Deaclan FinchPlace 25. · Grade 12 · Middleburg | 12 | Middleburg | 56.98a PR |
| 26. | Isaish BoatnerPlace 26. · Grade 11 · Clay | 11 | Clay | 57.09a |
| 27. | Elias LeePlace 27. · Grade 10 · Fleming Island | 10 | Fleming Island | 57.29a |
| 28. | Jermiah EasterlingPlace 28. · Grade 9 · Duval Charter | 9 | Duval Charter | 57.61a PR |
| 29. | Felipe VasquezPlace 29. · Grade 9 · Ridgeview | 9 | Ridgeview | 57.71a PR |
| 30. | Tucker PricePlace 30. · Grade 9 · Keystone Heights | 9 | Keystone Heights | 57.89a PR |
| 31. | Jayden MartinPlace 31. · Grade 9 · Clay | 9 | Clay | 58.06a |
| 32. | Gunner TranPlace 32. · Grade 11 · Duval Charter | 11 | Duval Charter | 58.13a PR |
| 33. | Corneilius NelsonPlace 33. · Grade 12 · Ridgeview | 12 | Ridgeview | 58.25a |
| 34. | Cameron HallPlace 34. · Grade 9 · Trinity Christian (Jacksonville) | 9 | Trinity Christian (Jacksonville) | 58.54a |
| 35. | Walter SullivanPlace 35. · Grade 9 · Madison County | 9 | Madison County | 59.13a |
| 36. | Conor DormanPlace 36. · Grade 9 · Clay | 9 | Clay | 59.34a |
| 37. | Brayden HigginbothamPlace 37. · Grade 11 · Baker County | 11 | Baker County | 59.45a |
| 38. | Andrew TylerPlace 38. · Grade 12 · Trinity Christian (Jacksonville) | 12 | Trinity Christian (Jacksonville) | 59.49a PR |
| 39. | Kiamsky CajourPlace 39. · Grade 11 · Orange Park | 11 | Orange Park | 59.51a |
| 40. | Kasir JenningPlace 40. · Grade 11 · Trinity Christian (Jacksonville) | 11 | Trinity Christian (Jacksonville) | 59.71a PR |
| 41. | Ryan HairstonPlace 41. · Grade 9 · Florida School for the Deaf & the Blind | 9 | Florida School for the Deaf & the Blind | 59.78a |
| 42. | Jakhi RhaneyPlace 42. · Grade 9 · Trinity Christian (Jacksonville) | 9 | Trinity Christian (Jacksonville) | 59.93a PR |
| 43. | Jayden RomanPlace 43. · Grade 10 · St Joseph Academy | 10 | St Joseph Academy | 1:00.30a |
| 44. | Seth FernandezPlace 44. · Grade 11 · Keystone Heights | 11 | Keystone Heights | 1:00.40a PR |
| 45. | Nicholas DeklePlace 45. · Grade 10 · Baker County | 10 | Baker County | 1:00.52a |
| 46. | Hunter TrouillePlace 46. · Grade 11 · Florida School for the Deaf & the Blind | 11 | Florida School for the Deaf & the Blind | 1:02.14a |
| 47. | Eli DarrasPlace 47. · Grade 12 · Baker County | 12 | Baker County | 1:02.25a PR |
| 48. | Elijah CrookPlace 48. · Grade 12 · Florida School for the Deaf & the Blind | 12 | Florida School for the Deaf & the Blind | 1:02.71a |
| 49. | Mason PellicerPlace 49. · Grade 10 · St Joseph Academy | 10 | St Joseph Academy | 1:03.50a PR |
| 50. | Paul WiedemannPlace 50. · Grade 9 · University Christian | 9 | University Christian | 1:03.86a PR |
| 51. | Jacob BuenaflorPlace 51. · Grade 9 · St Joseph Academy | 9 | St Joseph Academy | 1:04.11a PR |
| 52. | Josiah CainesPlace 52. · Grade 10 · Columbia (Lake City) | 10 | Columbia (Lake City) | 1:05.76a |
| 53. | Kingston CarrolPlace 53. · Grade 9 · Bradford | 9 | Bradford | 1:07.60a |
| 54. | Najhari JohnsonPlace 54. · Grade 9 · Madison County | 9 | Madison County | 1:09.31a |
