| 1 | Riley FoxPlace 1 · Grade 8 · Evangelical Christian | 8 | Evangelical Christian | 24:48.65 |
| 2 | Sofia DiazPlace 2 · Grade 9 · Aubrey Rogers | 9 | Aubrey Rogers | 25:32.85 |
| 3 | Mila MarenoskiPlace 3 · Grade 10 · Aubrey Rogers | 10 | Aubrey Rogers | 26:18.69 |
| 4 | Nadia PhilippsPlace 4 · Grade 9 · Estero | 9 | Estero | 27:16.49 |
| 5 | Zoe CertaPlace 5 · Grade 9 · St John Neumann | 9 | St John Neumann | 27:27.80 |
| 6 | Addison RoyerPlace 6 · Grade 10 · Bishop Verot | 10 | Bishop Verot | 27:48.05 PR |
| 7 | Melinda CruzPlace 7 · Grade 8 · Everglades City | 8 | Everglades City | 27:53.53 |
| 8 | Taylor SmithPlace 8 · Grade 11 · Oasis | 11 | Oasis | 27:57.04 |
| 9 | Claire ZyndaPlace 9 · Grade 9 · St John Neumann | 9 | St John Neumann | 28:36.79 |
| 10 | Shagun PatelPlace 10 · Grade 12 · Gulf Coast | 12 | Gulf Coast | 28:45.98 PR |
| 11 | Jacqueline BowersPlace 11 · Grade 12 · Estero | 12 | Estero | 29:10.10 |
| 12 | Allison BaackPlace 12 · Grade 6 · Evangelical Christian | 6 | Evangelical Christian | 29:19.90 |
| 13 | Jaidyn SweazyPlace 13 · Grade 12 · Lely | 12 | Lely | 29:57.58 |
| 14 | Leila ArshedPlace 14 · Grade 9 · Aubrey Rogers | 9 | Aubrey Rogers | 30:03.93 |
| 15 | Lina CatonPlace 15 · Grade 9 · St John Neumann | 9 | St John Neumann | 30:36.75 |
| 16 | Jocelyn CruzPlace 16 · Grade 11 · Gulf Coast | 11 | Gulf Coast | 30:48.24 |
| 17 | Estella TemkinPlace 17 · Grade 8 · Babcock | 8 | Babcock | 30:54.46 |
| 18 | Isabella PierrePlace 18 · Grade 10 · Bishop Verot | 10 | Bishop Verot | 30:56.27 |
| 19 | Addison StonePlace 19 · Grade 12 · Estero | 12 | Estero | 31:26.37 |
| 20 | Emily WisnerPlace 20 · Grade 6 · Evangelical Christian | 6 | Evangelical Christian | 31:29.83 |
| 21 | Alexis HerringPlace 21 · Grade 11 · Gulf Coast | 11 | Gulf Coast | 31:29.94 |
| 22 | Amber StecPlace 22 · Grade 10 · Gulf Coast | 10 | Gulf Coast | 31:40.45 |
| 23 | Emma MooneyPlace 23 · Grade 10 · Evangelical Christian | 10 | Evangelical Christian | 32:36.17 |
| 24 | Ireland SmithPlace 24 · Grade 9 · Gulf Coast | 9 | Gulf Coast | 32:51.19 |
| 25 | Stella LaFountainPlace 25 · Grade 10 · Babcock | 10 | Babcock | 34:04.78 PR |
| 26 | Riley SmithPlace 26 · Grade 10 · St John Neumann | 10 | St John Neumann | 34:05.20 |
| 27 | Ava ErazoPlace 27 · Grade 11 · Gulf Coast | 11 | Gulf Coast | 35:12.25 PR |
| 28 | Sarah GonzalezPlace 28 · Grade 10 · Bishop Verot | 10 | Bishop Verot | 35:14.67 |
| 29 | Brooke BricklerPlace 29 · Grade 10 · Oasis | 10 | Oasis | 35:20.96 |
| 30 | Sara GouahawPlace 30 · Grade 10 · Evangelical Christian | 10 | Evangelical Christian | 35:28.87 |
| 31 | Ruth CifuentesPlace 31 · Grade 10 · Everglades City | 10 | Everglades City | 36:09.37 PR |
| 32 | Lina PrietoPlace 32 · Grade 12 · Lely | 12 | Lely | 36:14.91 |
| 33 | Malia NilandPlace 33 · Grade 11 · Oasis | 11 | Oasis | 36:37.49 |
| 34 | Alaina BolandPlace 34 · Grade 9 · Gulf Coast | 9 | Gulf Coast | 36:41.15 |
| 35 | Macey HodgesPlace 35 · Grade 12 · North Fort Myers | 12 | North Fort Myers | 37:12.30 |
| 36 | Claire XuPlace 36 · Grade 11 · Evangelical Christian | 11 | Evangelical Christian | 38:19.49 |
| 37 | Zoe BaronPlace 37 · Grade 9 · Labelle | 9 | Labelle | 38:46.05 |
| 38 | Annie SoukupPlace 38 · Grade 12 · Babcock | 12 | Babcock | 43:01.21 PR |
