| 1 | George EscobarPlace 1 · Maclay Schoo | — | Maclay Schoo | 51.34 | 10 |
| 2 | Cordae JonesPlace 2 · Marianna | — | Marianna | 53.52 | 8 |
| 3 | Joshua PatriotisPlace 3 · Franklin Cou | — | Franklin Cou | 54.73 | 6 |
| 4 | Daniel GhantPlace 4 · Bay | — | Bay | 55.17 | 5 |
| 5 | Ashton HowellPlace 5 · Graceville H | — | Graceville H | 55.35 | 4 |
| 6 | Scottie PutneyPlace 6 · Bay | — | Bay | 55.40 | 3 |
| 7 | Marquise StaffordPlace 7 · Mosley | — | Mosley | 56.53 | 2 |
| 8 | Armon ButlerPlace 8 · Marianna | — | Marianna | 56.89 | 1 |
| 9 | Malik GibsonPlace 9 · Rutherford H | — | Rutherford H | 57.31 | 3 |
| 10 | Justin ReidPlace 10 · Bay | — | Bay | 57.38 | 3 |
| 11 | Noah DevillePlace 11 · Bay | — | Bay | 57.48 | 4 |
| 12 | Leon VilardiPlace 12 · North Bay Ha | — | North Bay Ha | 59.19 | 5 |
| 13 | Jadarius GrayPlace 13 · Graceville H | — | Graceville H | 59.76 | 5 |
| 14 | Cameron WilliamsPlace 14 · Famu High Sc | — | Famu High Sc | 59.85 | 2 |
| 15 | cody meeksPlace 15 · North Bay Ha | — | North Bay Ha | 1:00.16 | 3 |
| 16 | Darian VaughnPlace 16 · Mosley | — | Mosley | 1:00.42 | 2 |
| 17 | Adrian AndrewsPlace 17 · Famu High Sc | — | Famu High Sc | 1:00.85 | 6 |
| 18 | Cayden WoodsPlace 18 · Maclay Schoo | — | Maclay Schoo | 1:02.94 | 5 |
| 19 | Tyson MarlowPlace 19 · Mosley | — | Mosley | 1:03.02 | 2 |
| 20 | Colin McDuffiePlace 20 · Maclay Schoo | — | Maclay Schoo | 1:04.56 | 5 |
| 21 | Damien PenderPlace 21 · Marianna | — | Marianna | 1:04.80 | 1 |
| 22 | Austin McKeePlace 22 · Franklin Cou | — | Franklin Cou | 1:04.99 | 3 |
| 23 | Joseph McCannPlace 23 · Maclay Schoo | — | Maclay Schoo | 1:05.45 | 4 |
| 24 | Charlie NicholsPlace 24 · Graceville H | — | Graceville H | 1:05.46 | 5 |
| 25 | Sion GroganPlace 25 · North Bay Ha | — | North Bay Ha | 1:06.75 | 1 |
| 26 | Tyrell RobinsonPlace 26 · North Bay Ha | — | North Bay Ha | 1:07.17 | 4 |
| 27 | Cavante JacksonPlace 27 · Mosley | — | Mosley | 1:08.67 | 2 |
| 28 | Ezekiel BlountPlace 28 · Marianna | — | Marianna | 1:10.17 | 1 |
| 29 | Colby QuinnPlace 29 · Port St. Joe | — | Port St. Joe | 1:12.58 | 5 |
