| 1 | Bridget FitzsimonPlace 1 · Paul VI | — | Paul VI | 43.36 | 1 |
| 2 | Bijee JacksonPlace 2 · Episcopal | — | Episcopal | 43.93 | 2 |
| 3 | Dawn FarquharsonPlace 3 · Episcopal | — | Episcopal | 44.06 | 1 |
| 4 | Zoe BelodeauPlace 4 · St Stephens | — | St Stephens | 45.33 | 3 |
| 5 | Jenna CooperPlace 5 · Paul VI | — | Paul VI | 45.50 | 3 |
| 6 | Annie PeartreePlace 6 · Sidwell Frie | — | Sidwell Frie | 45.75 | 2 |
| 7 | Lathisa DaalPlace 7 · Saint John P | — | Saint John P | 45.78 | 1 |
| 8 | Katherine TaylorPlace 8 · Saint John P | — | Saint John P | 46.98 | 5 |
| 9 | Abby PogrebaPlace 9 · Paul VI | — | Paul VI | 47.77 | 3 |
| 10 | Caroline HarmsPlace 10 · Saint John P | — | Saint John P | 47.83 | 5 |
| 11 | Annie LederPlace 11 · Georgetown D | — | Georgetown D | 48.17 | 6 |
| 12 | Carolina VitovitskyPlace 12 · Saint John P | — | Saint John P | 48.48 | 4 |
| 13 | Alexandra WatsonPlace 13 · Georgetown V | — | Georgetown V | 48.55 | 4 |
| 14 | Makaela EdmondsPlace 14 · Paul VI | — | Paul VI | 48.96 | 5 |
| 15 | Elise HellmannPlace 15 · St Stephens | — | St Stephens | 49.09 | 7 |
| 16 | Iyanna LewisPlace 16 · Sidwell Frie | — | Sidwell Frie | 49.10 | 6 |
| 17 | Catie StackPlace 17 · Flint Hill | — | Flint Hill | 49.28 | 11 |
| 18 | Logan TylerPlace 18 · Episcopal | — | Episcopal | 49.68 | 6 |
| 19 | Ellie LeapePlace 19 · Sidwell Frie | — | Sidwell Frie | 49.70 | 5 |
| 20 | Maddie BlalockPlace 20 · Episcopal | — | Episcopal | 49.80 | 6 |
| 21 | Kate CowardPlace 21 · St Stephens | — | St Stephens | 50.19 | 9 |
| 22 | Sunny MillerPlace 22 · Episcopal | — | Episcopal | 50.24 | 8 |
| 23 | Haley PrincePlace 23 · Potomac Scho | — | Potomac Scho | 51.07 | 10 |
| 24 | Macy YoungPlace 24 · Episcopal | — | Episcopal | 51.31 | 7 |
| 25 | Allison McKenziePlace 25 · Georgetown V | — | Georgetown V | 51.67 | 11 |
| 26 | Michelle NicholsPlace 26 · Georgetown D | — | Georgetown D | 51.70 | 8 |
| 27 | Sara O'BrienPlace 27 · Georgetown V | — | Georgetown V | 52.43 | 12 |
| 28 | Jill GollobPlace 28 · Georgetown V | — | Georgetown V | 52.49 | 12 |
| 29 | Abby BlascovichPlace 29 · Paul VI | — | Paul VI | 52.50 | 4 |
| 30 | Maggie MattoxPlace 30 · Episcopal | — | Episcopal | 52.60 | 10 |
| 31 | Calia LeePlace 31 · Georgetown V | — | Georgetown V | 52.95 | 5 |
| 32 | Rachel DavisPlace 32 · Paul VI | — | Paul VI | 53.02 | 2 |
| 33 | Halle KnutsonPlace 33 · St Stephens | — | St Stephens | 53.05 | 9 |
| 34 | Caroline HullmanPlace 34 · Potomac Scho | — | Potomac Scho | 53.41 | 11 |
| 35 | Mary Claire MadisonPlace 35 · Saint John P | — | Saint John P | 53.70 | 9 |
| 36 | Sara ZarghamPlace 36 · Georgetown D | — | Georgetown D | 53.87 | 7 |
| 37 | Alexa RooneyPlace 37 · Potomac Scho | — | Potomac Scho | 54.01 | 10 |
| 38 | Kaycie WangPlace 38 · Episcopal | — | Episcopal | 54.17 | 8 |
| 39 | Bridget LuttonPlace 39 · Saint John P | — | Saint John P | 54.70 | 8 |
| 40 | Cecilia MoorePlace 40 · Episcopal | — | Episcopal | 54.99 | 10 |
| 41 | Kate CastlePlace 41 · Episcopal | — | Episcopal | 55.04 | 9 |
| 42 | Bridget HanleyPlace 42 · St Stephens | — | St Stephens | 55.21 | 10 |
| 43 | Bea CampagnaPlace 43 · Episcopal | — | Episcopal | 55.34 | 11 |
| 44 | Helen HoranPlace 44 · Potomac Scho | — | Potomac Scho | 55.38 | 9 |
| 45 | Angela ZhengPlace 45 · Madeira | — | Madeira | 55.98 | 12 |
| 46 | Ashley Van HornePlace 46 · St Stephens | — | St Stephens | 57.97 | 11 |
| 47 | Valerie YuanPlace 47 · Madeira | — | Madeira | 1:00.11 | 12 |