| 1 | Jayden ReyesPlace 1 · Grade JR · Cape Coral | JR | Cape Coral | 49.14 |
| 2 | Jadrien CarmoPlace 2 · Grade JR · Bishop Verot | JR | Bishop Verot | 50.40 |
| 3 | Jack GiffordPlace 3 · Grade SR · Southwest Florida Christi | SR | Southwest Florida Christi | 50.59 |
| 4 | Ken'Dahrius GreenPlace 4 · Grade SR · Dunbar | SR | Dunbar | 50.90 |
| 5 | Wilkens SamediPlace 5 · Grade SR · Cypress Lake | SR | Cypress Lake | 51.08 |
| 6 | John RuggieroPlace 6 · Grade SR · Gulf Coast | SR | Gulf Coast | 51.26 |
| 7 | Bruno MenezesPlace 7 · Grade SO · Cypress Lake | SO | Cypress Lake | 51.37 |
| 8 | Peyton CarterPlace 8 · Grade SR · Bishop Verot | SR | Bishop Verot | 51.62 |
| 9 | Ethan HutchesonPlace 9 · Grade SR · Fort Myers | SR | Fort Myers | 52.02 |
| 10 | Marcelo ProenzaPlace 10 · Grade JR · Island Coast | JR | Island Coast | 52.18 |
| 11 | D'Mauri FrancoisPlace 11 · Grade JR · Dunbar | JR | Dunbar | 52.41 |
| 12 | Ivan RomanPlace 12 · Grade JR · Ida Baker | JR | Ida Baker | 52.85 |
| 13 | Leonel LatusPlace 13 · Grade JR · South Fort Myers | JR | South Fort Myers | 53.05 |
| 14 | Esdres LouisPlace 14 · Grade SR · Dunbar | SR | Dunbar | 53.06 |
| 15 | joel santanaPlace 15 · Grade SR · Cypress Lake | SR | Cypress Lake | 53.22 |
| 16 | Brandon HallPlace 16 · Grade JR · Bishop Verot | JR | Bishop Verot | 53.35 |
| 17 | Ceasar LaraPlace 17 · Grade JR · Gulf Coast | JR | Gulf Coast | 53.37 |
| 18 | Landon UngerPlace 18 · Grade SR · Island Coast | SR | Island Coast | 53.62 |
| 19 | Mason JessognePlace 19 · Grade SR · Canterbury (FM) | SR | Canterbury (FM) | 53.67 |
| 20 | Peyton SagessePlace 20 · Grade SO · Gulf Coast | SO | Gulf Coast | 53.69 |
| 21 | Alex BeckerPlace 21 · Grade SR · Barron Collier | SR | Barron Collier | 53.70 |
| 22 | Kemoni BrooksPlace 22 · Grade SO · East Lee County | SO | East Lee County | 53.82 |
| 23 | Michael ButlerPlace 23 · Grade JR · South Fort Myers | JR | South Fort Myers | 53.88 |
| 24 | Osmani ZayasPlace 24 · Grade SO · Ida Baker | SO | Ida Baker | 54.04 |
| 25 | Roman AvilesPlace 25 · Grade JR · North Fort Myers | JR | North Fort Myers | 54.20 |
| 26 | Joel GiardettiPlace 26 · Grade JR · Southwest Florida Christi | JR | Southwest Florida Christi | 54.40 |
| 27 | Campbell SamsPlace 27 · Grade SR · Fort Myers | SR | Fort Myers | 54.47 |
| 28 | Becket AllanPlace 28 · Grade SO · Aubrey Rogers | SO | Aubrey Rogers | 54.50 |
| 29 | Jose Paz TeranPlace 29 · Grade SR · Fort Myers | SR | Fort Myers | 54.51 |
| 30 | Logan PaulPlace 30 · Grade SO · Palmetto Ridge | SO | Palmetto Ridge | 54.68 |
| 31 | Jaiden BayleyPlace 31 · Grade JR · Ida Baker | JR | Ida Baker | 54.89 |
| 32 | Todrick LanniganPlace 32 · Grade SO · East Lee County | SO | East Lee County | 55.05 |
| 33 | Julian BriscoPlace 33 · Grade FR · Evangelical Christian | FR | Evangelical Christian | 55.07 |
| 34 | Benjamin PerryPlace 34 · Grade SR · Naples | SR | Naples | 55.22 |
| 35 | Eliyahu CheryPlace 35 · Grade JR · Naples | JR | Naples | 55.41 |
| 36 | Bryce NapierPlace 36 · Grade SR · North Fort Myers | SR | North Fort Myers | 55.44 |
| 37 | Ben PetersPlace 37 · Grade 8 · Evangelical Christian | 8 | Evangelical Christian | 55.68 |
| 38 | Jaden WickmanPlace 38 · Grade SO · Evangelical Christian | SO | Evangelical Christian | 55.83 |
| 39 | James HallPlace 39 · Grade JR · Cape Coral | JR | Cape Coral | 55.90 |
| 40 | Wedsen LoristonPlace 40 · Grade SO · Lehigh | SO | Lehigh | 56.61 |
| 41 | Godson CharlesPlace 41 · Grade JR · Lehigh | JR | Lehigh | 56.93 |
| 42 | David AmayaPlace 42 · Grade JR · Immokalee | JR | Immokalee | 57.06 |
| 43 | Orlando CamachoPlace 43 · Grade SO · LaBelle | SO | LaBelle | 57.14 |
| 44 | Gabe DahanPlace 44 · Grade SR · Canterbury (FM) | SR | Canterbury (FM) | 57.19 |
| 45 | Benjamin PucinPlace 45 · Grade FR · Mariner | FR | Mariner | 57.66 |
| 46 | John BrackinPlace 46 · Grade SR · Barron Collier | SR | Barron Collier | 57.67 |
| 47 | Sorek SecorePlace 47 · Grade FR · Barron Collier | FR | Barron Collier | 57.68 |
| 48 | Javirin SingletonPlace 48 · Grade JR · East Lee County | JR | East Lee County | 57.87 |
| 49 | Brody ProutPlace 49 · Grade SO · Babcock | SO | Babcock | 58.43 |
| 50 | Riffordigno RegistrePlace 50 · Grade SR · Immokalee | SR | Immokalee | 58.44 |
| 51 | Javier ParodisPlace 51 · Grade FR · Aubrey Rogers | FR | Aubrey Rogers | 58.55 |
| 52 | Nehemiah MullingsPlace 52 · Grade SR · Clewiston | SR | Clewiston | 58.56 |
| 53 | Tondrick MackPlace 53 · Grade SO · Island Coast | SO | Island Coast | 58.70 |
| 54 | Johnny AshPlace 54 · Grade SR · Lehigh | SR | Lehigh | 58.79 |
| 55 | Francisco RosasPlace 55 · Grade JR · LaBelle | JR | LaBelle | 59.41 |
| 56 | Gladwin AugustinPlace 56 · Grade JR · Riverdale | JR | Riverdale | 59.53 |
| 57 | Nicholas GarciaPlace 57 · Grade SO · North Fort Myers | SO | North Fort Myers | 59.78 |
| 58 | Ian BreaPlace 58 · Grade JR · Palmetto Ridge | JR | Palmetto Ridge | 1:00.04 |
| 59 | Richard WaddellPlace 59 · Grade JR · Mariner | JR | Mariner | 1:00.10 |
| 60 | Jan-Franco OrtizPlace 60 · Grade FR · Naples | FR | Naples | 1:01.23 |
| 61 | Wyatt TresselPlace 61 · Grade FR · Babcock | FR | Babcock | 1:01.38 |
| 62 | Sawyer NassPlace 62 · Grade SO · Riverdale | SO | Riverdale | 1:01.68 |
| 63 | Trey McCainPlace 63 · Grade SR · Clewiston | SR | Clewiston | 1:01.86 |
| 64 | Nash AkersPlace 64 · Grade 8 · Southwest Florida Christi | 8 | Southwest Florida Christi | 1:02.02 |
| 65 | Dillon LundyPlace 65 · Grade SO · Babcock | SO | Babcock | 1:02.64 |
| 66 | Kalib SimmonsPlace 66 · Grade SR · LaBelle | SR | LaBelle | 1:02.98 |
| 67 | Santiago PhilippsPlace 67 · Grade SO · Estero | SO | Estero | 1:05.19 |
| 68 | Ja'yln MillerPlace 68 · Grade SR · Clewiston | SR | Clewiston | 1:08.79 |
| 69 | NIcolas FrangelloPlace 69 · Grade FR · Estero | FR | Estero | 1:08.93 |
