| 1 | Abia KantePlace 1 · Grade SO · Cumberlands | SO | Cumberlands | 23.83 |
| 2 | Aaliyannah AndersonPlace 2 · Grade SO · Lincoln (Mo.) | SO | Lincoln (Mo.) | 24.39 |
| 3 | Zakiya PorterPlace 3 · Grade JR · Central Methodist | JR | Central Methodist | 24.46 |
| 4 | Joana VakasobuduruPlace 4 · Grade JR · Central Methodist | JR | Central Methodist | 24.48 |
| 5 | Lillian GuzmanPlace 5 · Grade SO · Cumberlands | SO | Cumberlands | 24.68 |
| 6 | Namibia ClavierPlace 6 · Grade SR · Lincoln (Mo.) | SR | Lincoln (Mo.) | 24.76 |
| 7 | Dayana MontenegroPlace 7 · Central Running Club | — | Central Running Club | 24.95 |
| 8 | Denita JacksonPlace 8 · Grade SR · Lincoln (Mo.) | SR | Lincoln (Mo.) | 25.01 |
| 9 | Diane OndoboPlace 9 · Grade JR · Central Methodist | JR | Central Methodist | 25.12 |
| 10 | Daizy SolirPlace 10 · Grade SR · MidAmerica Nazarene | SR | MidAmerica Nazarene | 25.48 |
| 11 | Aroa CarballoPlace 11 · Grade FR · Central Methodist | FR | Central Methodist | 25.51 |
| 12 | Parys SmithPlace 12 · Grade SR · Central Methodist | SR | Central Methodist | 25.61 |
| 13 | Monea CareyPlace 13 · Grade SR · Lincoln (Mo.) | SR | Lincoln (Mo.) | 25.65 |
| 14 | J'Nya DemmingPlace 14 · Grade SO · Highland CC | SO | Highland CC | 25.70 |
| 15 | Renee FinkePlace 15 · Grade SR · Central Methodist | SR | Central Methodist | 25.85 |
| 16 | Alexia LewisPlace 16 · Grade SO · Highland CC | SO | Highland CC | 25.92 |
| 17 | Jola FelixPlace 17 · Grade SO · Highland CC | SO | Highland CC | 25.99 |
| 18 | Abigail BrooksPlace 18 · Grade SR · Lincoln (Mo.) | SR | Lincoln (Mo.) | 26.23 |
| 19 | Brooklyn JohnsonPlace 19 · Grade JR · Mount Mercy | JR | Mount Mercy | 26.35 |
| 20 | Zyaire RobinsonPlace 20 · Grade JR · Central Methodist | JR | Central Methodist | 26.37 |
| 21 | Madison DixonPlace 21 · Grade JR · William Woods | JR | William Woods | 26.40 |
| 22 | Allie BrysonPlace 22 · Grade SO · Cumberlands | SO | Cumberlands | 26.45 |
| 23 | Joslyn GriffinPlace 23 · Grade FR · MidAmerica Nazarene | FR | MidAmerica Nazarene | 26.59 |
| 24 | Jolee MeszPlace 24 · Grade SO · Morningside | SO | Morningside | 26.87 |
| 25 | Sofia RizoPlace 25 · Grade FR · Central Methodist | FR | Central Methodist | 26.90 |
| 26 | Krystina ClemonsPlace 26 · Grade SO · Highland CC | SO | Highland CC | 26.93 |
| 27 | Kianna PattonPlace 27 · Grade SR · Webster | SR | Webster | 27.02 |
| 28 | Jesse GreshamPlace 28 · Grade FR · William Woods | FR | William Woods | 27.21 |
| 29 | Sydney BrouwerPlace 29 · Grade FR · Morningside | FR | Morningside | 27.33 |
| 30 | Morgan SachsPlace 30 · Grade FR · Morningside | FR | Morningside | 27.40 |
| 31 | Allisa WardPlace 31 · Grade SR · MidAmerica Nazarene | SR | MidAmerica Nazarene | 27.61 |
| 32 | Sakoya JohnsonPlace 32 · Grade JR · Central Methodist | JR | Central Methodist | 27.67 |
| 33 | Meredith DraperPlace 33 · Grade SO · Morningside | SO | Morningside | 27.69 |
| 34 | Catie ReittingerPlace 34 · Grade FR · Mount Mercy | FR | Mount Mercy | 27.73 |
| 35 | Hannah EdwardsPlace 35 · Grade FR · MidAmerica Nazarene | FR | MidAmerica Nazarene | 27.79 |
| 36 | Samantha BitzPlace 36 · Grade JR · Missouri Valley | JR | Missouri Valley | 27.96 |
| 37 | Kirsten WetterstromPlace 37 · Grade FR · MidAmerica Nazarene | FR | MidAmerica Nazarene | 28.17 |
| 38 | Aissata KamaraPlace 38 · Grade JR · Webster | JR | Webster | 28.28 |
| 39 | Sheridan UtleyPlace 39 · Grade FR · Central Methodist | FR | Central Methodist | 28.47 |
| 40 | Tabitha RogersPlace 40 · Grade SR · William Penn | SR | William Penn | 29.40 |
| 41 | Aaliyah LopezPlace 41 · Grade FR · William Penn | FR | William Penn | 29.70 |
| 42 | Grace TestermanPlace 42 · Grade JR · Graceland | JR | Graceland | 30.37 |
| 43 | Maurkevia PettusPlace 43 · Grade FR · Graceland | FR | Graceland | 34.01 |
