| 1 | Lizzy McCannPlace 1 · Grade 12 · Van Meter | 12 | Van Meter | 2:29.47a |
| 2 | Madelyn GrothusPlace 2 · Grade 11 · Madrid | 11 | Madrid | 2:32.77a |
| 3 | Lydia HunterPlace 3 · Grade 11 · Van Meter | 11 | Van Meter | 2:40.02a |
| 4 | Tatum FriedrichPlace 4 · Grade 11 · Mount Ayr | 11 | Mount Ayr | 2:42.20a |
| 5 | Kaitlyn HickmanPlace 5 · Grade 9 · Atlantic | 9 | Atlantic | 2:42.72a |
| 6 | Kinsley CanoyerPlace 6 · Grade 10 · Earlham | 10 | Earlham | 2:43.39a |
| 7 | Adysen ChurchillPlace 7 · Grade 12 · Woodward-Granger | 12 | Woodward-Granger | 2:44.05a |
| 8 | Megan UnderwoodPlace 8 · Grade 10 · ACGC | 10 | ACGC | 2:44.32a |
| 9 | Laicey LutzPlace 9 · Grade 11 · Panorama Panora | 11 | Panorama Panora | 2:45.28a |
| 10 | Addison DoolittlePlace 10 · Grade 12 · Mount Ayr | 12 | Mount Ayr | 2:46.10a |
| 11 | Olivia PudenzPlace 11 · Grade 10 · Madrid | 10 | Madrid | 2:51.66a |
| 12 | Brooklyn SchaferPlace 12 · Grade 11 · ACGC | 11 | ACGC | 2:52.66a |
| 13 | Layah AndersonPlace 13 · Grade 10 · Earlham | 10 | Earlham | 2:55.46a |
| 14 | Brynnlee SeeckPlace 14 · Grade 10 · ACGC | 10 | ACGC | 2:56.62a |
| 15 | Kate NewtonPlace 15 · Grade 10 · Mount Ayr | 10 | Mount Ayr | 2:56.67a |
| 16 | Alex LeitingPlace 16 · Grade 11 · St. Edmond Fort Dodge | 11 | St. Edmond Fort Dodge | 2:57.99a |
| 17 | Naomi VallesPlace 17 · Grade 10 · St. Edmond Fort Dodge | 10 | St. Edmond Fort Dodge | 2:58.76a |
| 18 | Lily EslickPlace 18 · Grade 11 · St. Edmond Fort Dodge | 11 | St. Edmond Fort Dodge | 3:00.67a |
| 19 | Mia LindseyPlace 19 · Van Meter | — | Van Meter | 3:01.52a |
| 20 | Corrie KnappPlace 20 · Grade 10 · Panorama Panora | 10 | Panorama Panora | 3:03.97a |
| 21 | Adelyn BangertPlace 21 · St. Edmond Fort Dodge | — | St. Edmond Fort Dodge | 3:14.60a |
| 22 | Milena KellerPlace 22 · Madrid | — | Madrid | 3:17.75a |
| 23 | Kacie LarsenPlace 23 · Grade 12 · Mount Ayr | 12 | Mount Ayr | 3:19.65a |
| 24 | Landrie LightPlace 24 · Grade 9 · West Central Valley Stuart | 9 | West Central Valley Stuart | 3:23.35a |
| 25 | Lilyen ChurchillPlace 25 · Grade 10 · Woodward-Granger | 10 | Woodward-Granger | 3:24.42a |
| 26 | Gwen Osborn-SimmondsPlace 26 · Grade 10 · Greene County | 10 | Greene County | 3:26.29a |
| 27 | Chelsea KopaskaPlace 27 · Grade 12 · ACGC | 12 | ACGC | 3:29.65a |
| 28 | Elyzah OlsonPlace 28 · Grade 10 · West Central Valley Stuart | 10 | West Central Valley Stuart | 4:20.06a |
| 1 | Van MeterPlace 1 · Van Meter | — | Van Meter | 1:54.40a |
| 2 | St. Edmond Fort DodgePlace 2 · St. Edmond Fort Dodge | — | St. Edmond Fort Dodge | 1:57.20a |
| 3 | Mount AyrPlace 3 · Mount Ayr | — | Mount Ayr | 1:59.45a |
| 4 | Greene CountyPlace 4 · Greene County | — | Greene County | 2:01.54a |
| 5 | EarlhamPlace 5 · Earlham | — | Earlham | 2:03.47a |
| 6 | ACGCPlace 6 · ACGC | — | ACGC | 2:03.82a |
| 7 | AtlanticPlace 7 · Atlantic | — | Atlantic | 2:07.96a |
| 8 | MadridPlace 8 · Madrid | — | Madrid | 2:10.29a |
| 9 | West Central Valley StuartPlace 9 · West Central Valley Stuart | — | West Central Valley Stuart | 2:11.21a |
| 10 | EarlhamPlace 10 · Earlham | — | Earlham | 2:14.94a |
| — | Woodward-Granger · Woodward-Granger | — | Woodward-Granger | DQ |
