| 1 | Bongumusa NkosiPlace 1 · Iowa Central CC | — | Iowa Central CC | 47.76a |
| 2 | Nathan SimonsPlace 2 · Sioux City Track Club | — | Sioux City Track Club | 48.24a |
| 3 | Isaac DavelaarPlace 3 · Dordt (Iowa) | — | Dordt (Iowa) | 48.45a |
| 4 | Zavio PratoPlace 4 · Mount Marty (S.D.) | — | Mount Marty (S.D.) | 48.77a |
| 5 | Christopher CormierPlace 5 · Chadron State | — | Chadron State | 49.20a |
| 6 | Braden PetersonPlace 6 · South Dakota State | — | South Dakota State | 49.28a |
| 7 | Nicquaine HenryPlace 7 · Chadron State | — | Chadron State | 49.34a |
| 8 | Seth MartensPlace 8 · Northern State | — | Northern State | 49.35a |
| 9 | Drew SalfrankPlace 9 · Northern State | — | Northern State | 49.43a |
| 10 | Tony WilliamsPlace 10 · Iowa Central CC | — | Iowa Central CC | 49.64a |
| 11 | Lucas FlemmerPlace 11 · South Dakota State | — | South Dakota State | 49.89a |
| 12 | Quentin LewisPlace 12 · Dickinson State (N.D.) | — | Dickinson State (N.D.) | 49.91a |
| 13 | Jeremiah DonahoePlace 13 · South Dakota State | — | South Dakota State | 50.17a |
| 14 | Noah SchroderPlace 14 · Dordt (Iowa) | — | Dordt (Iowa) | 50.20a |
| 15 | Kadin PerezPlace 15 · Chadron State | — | Chadron State | 50.30a |
| 16 | Franklin JohnsPlace 16 · South Dakota State | — | South Dakota State | 50.51a |
| 17 | Reggie SlabaPlace 17 · South Dakota State | — | South Dakota State | 50.64a |
| 18 | Ethan PitlickPlace 18 · South Dakota Mines | — | South Dakota Mines | 50.79a |
| 19 | Carl MassaPlace 19 · Mount Marty (S.D.) | — | Mount Marty (S.D.) | 50.82a |
| 20 | Nate McCormickPlace 20 · South Dakota State | — | South Dakota State | 51.13a |
| 21 | Eddie JohnsonPlace 21 · South Dakota Mines | — | South Dakota Mines | 51.22a |
| 22 | Cameron JonesPlace 22 · Iowa Central CC | — | Iowa Central CC | 51.34a |
| 23 | Dawson TeunePlace 23 · Dordt (Iowa) | — | Dordt (Iowa) | 51.47a |
| 24 | Aaron MasengePlace 24 · Iowa Western CC | — | Iowa Western CC | 51.52a |
| 25 | Scott KrollPlace 25 · Dordt (Iowa) | — | Dordt (Iowa) | 51.72a |
| 26 | Brayden HoulettePlace 26 · South Dakota State | — | South Dakota State | 51.77a |
| 27 | Nate PattersonPlace 27 · South Dakota State | — | South Dakota State | 51.93a |
| 28 | Carson ElbersPlace 28 · Dordt (Iowa) | — | Dordt (Iowa) | 51.93a |
| 29 | Payton AndersonPlace 29 · South Dakota State | — | South Dakota State | 51.94a |
| 30 | Melik DavisPlace 30 · Augustana (S.D.) | — | Augustana (S.D.) | 52.14a |
| 31 | Dylan ObbinkPlace 31 · Dordt (Iowa) | — | Dordt (Iowa) | 52.16a |
| 32 | Malachi SwallowPlace 32 · Chadron State | — | Chadron State | 52.39a |
| 33 | Gage PlaggePlace 33 · Northwestern (Iowa) | — | Northwestern (Iowa) | 52.42a |
| 34 | Jacob JeffersonPlace 34 · Chadron State | — | Chadron State | 52.52a |
| 35 | Adam FillipiPlace 35 · Morningside (Iowa) | — | Morningside (Iowa) | 52.79a |
| 36 | AJ BomgaarsPlace 36 · Northwestern (Iowa) | — | Northwestern (Iowa) | 52.84a |
| 37 | Carl AskinsPlace 37 · Dickinson State (N.D.) | — | Dickinson State (N.D.) | 52.92a |
| 38 | Landon MeerdinkPlace 38 · Dordt (Iowa) | — | Dordt (Iowa) | 52.93a |
| 39 | Maximus KadrmasPlace 39 · Dickinson State (N.D.) | — | Dickinson State (N.D.) | 52.98a |
| 40 | Jack KlingPlace 40 · Morningside (Iowa) | — | Morningside (Iowa) | 53.04a |
| 41 | Caston FrankPlace 41 · Iowa Central CC | — | Iowa Central CC | 53.19a |
| 42 | Hayden LockiePlace 42 · Dickinson State (N.D.) | — | Dickinson State (N.D.) | 53.31a |
| 43 | Caden SwobodaPlace 43 · Crown | — | Crown | 53.44a |
| 44 | Elias JensenPlace 44 · Northwestern (Iowa) | — | Northwestern (Iowa) | 53.50a |
| 45 | Sunel FernandezPlace 45 · Waldorf (Iowa) | — | Waldorf (Iowa) | 53.67a |
| 46 | Jarett SchuchardPlace 46 · Dickinson State (N.D.) | — | Dickinson State (N.D.) | 53.96a |
| 47 | Dillon CottonPlace 47 · Iowa Central CC | — | Iowa Central CC | 53.98a |
| 48 | Jaimere CampbellPlace 48 · Iowa Central CC | — | Iowa Central CC | 54.07a |
| 49 | Deven NixonPlace 49 · Iowa Central CC | — | Iowa Central CC | 54.31a |
| 50 | William Walther IIIPlace 50 · Mount Marty (S.D.) | — | Mount Marty (S.D.) | 54.56a |
| 51 | Chandler GrayPlace 51 · Crown | — | Crown | 54.61a |
| 52 | Dawson MillerPlace 52 · South Dakota State | — | South Dakota State | 54.69a |
| 53 | Mykel PaulsenPlace 53 · Unattached | — | Unattached | 55.69a |
| 54 | Coltin VargasPlace 54 · Northwestern (Iowa) | — | Northwestern (Iowa) | 55.92a |
| 55 | Quin PetersonPlace 55 · Crown | — | Crown | 57.57a |
| 56 | Levi LanglossPlace 56 · Mount Marty (S.D.) | — | Mount Marty (S.D.) | 57.60a |
| 57 | Caden OligarioPlace 57 · UNAT-Augustana (S.D.) | — | UNAT-Augustana (S.D.) | 57.64a |
| 58 | Bryant PaulsenPlace 58 · South Dakota State | — | South Dakota State | 57.79a |
| 59 | Michael WilliamsPlace 59 · Waldorf (Iowa) | — | Waldorf (Iowa) | 59.04a |
| 60 | Andrew JohnsonPlace 60 · Crown | — | Crown | 59.76a |
